Journal: Arthritis Research & Therapy

Article Title: Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis

doi: 10.1186/s13075-023-03243-z

Figure Lengend Snippet: Plasma concentration of SPP1, SAA1, and KNG1 in MDA5 + DM RP-ILD. A–C The concentrations of SPP1, SAA1, and KNG1 in the plasma from an independent cohort were determined by ELISA. P values were calculated by the ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. D–F ROC curve showed the AUC of the 3 verified proteins, respectively

Article Snippet: Following the manufacturer’s instructions, commercial ELISA kits were utilized for quantifying the levels of serum amyloid A1 (SAA1) (Boster Bio, China), V-Set And Immunoglobulin Domain Containing 4 (VSIG4), Coagulation Factor XI (F11) (both from CUSABIO, China), Coagulation Factor XIII A Chain (F13A1), Secreted phosphoprotein 1 (SPP1) and Kininogen 1 (KNG1) (all from Uscn life Science, China) in plasma samples from both patients and normal control subjects.

Techniques: Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Biomedicines

Article Title: Effect of N-3 Polyunsaturated Fatty Acids on Lipid Composition in Familial Hypercholesterolemia: A Randomized Crossover Trial

doi: 10.3390/biomedicines10081809

Figure Lengend Snippet: High-density lipoprotein function at baseline presented by sequence, treatment period, and total.

Article Snippet: The plasma SAA1 levels were measured by an enzyme-linked immunosorbent assay (DY3019-05, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Sequencing, Capillary Electrochromatography

Journal: Biomedicines

Article Title: Effect of N-3 Polyunsaturated Fatty Acids on Lipid Composition in Familial Hypercholesterolemia: A Randomized Crossover Trial

doi: 10.3390/biomedicines10081809

Figure Lengend Snippet: Apolipoproteins and high-density lipoprotein function presented by treatment.

Article Snippet: The plasma SAA1 levels were measured by an enzyme-linked immunosorbent assay (DY3019-05, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Capillary Electrochromatography

Journal: bioRxiv

Article Title: Serum Amyloid A promotes Acetaminophen-induced liver injury by damaging sinusoidal endothelial cell and exacerbating platelet aggregation in liver

doi: 10.1101/2021.12.01.470869

Figure Lengend Snippet: Mice were intraperitoneally injected with APAP (300 mg/kg), the liver tissues and serum were collected for further analysis. (A) Immunohistochemistry staining of Saa1/2 in liver sections; (B-C) Expression of Saa1/2 in liver tissues were detected by q-PCR (n=3-5) (B) or western blots (C); (D) The Saa1/2 level in serum was detected by ELISA (n=3-4); (E-F) Primary hepatocytes were isolated from normal mice that were treated with APAP (10 or 20 mM) for indicated times, the intracellular Saa1/2 level was detected by western blots (E), and the secretive Saa1/2 in hepatocyte culture medium was detected by ELISA (F) (n=3); (G) Primary hepatocytes were treated with Saa1/2 (mixture of Saa1 and Saa2) recombinant proteins for the indicated times, and Saa1/2 expression was measured by western blots. (H) Primary hepatocytes were treated with APAP (10 mM) or Saa1/2 (3 μg/ml) alone or mix together for 6 hours, and Saa1/2 level was measured by western blots (H). Scale bar = 75 μm. *, p < 0 . 05 .

Article Snippet: Western blotting was performed to detect the protein level of Saa1/2 (cat. ab124964; Abcam), β-actin (cat.4970; Cell Signaling Technology) and Vcam-1 (cat.32653S; Cell Signaling Technology).

Techniques: Injection, Immunohistochemistry, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Isolation, Recombinant

Journal: bioRxiv

Article Title: Serum Amyloid A promotes Acetaminophen-induced liver injury by damaging sinusoidal endothelial cell and exacerbating platelet aggregation in liver

doi: 10.1101/2021.12.01.470869

Figure Lengend Snippet: (A) Schematic of mice receiving anti-SAA neutralizing antibodies treatment one hour before APAP-induced mouse liver injury; (B) serum concentrations of ALT and AST from mice treated with IgG or anti-SAA neutralizing antibodies (n=3-4); (C) H&E staining images (scale bars = 100 μm); (D) quantification of liver necrosis areas in H&E staining sections (n=3-7); (E) Survival curves of mice treated with either IgG or neutralizing antibodies of Saa1/2 (anti-SAA, 5 μg/mice) one hour before intraperitoneal injection of APAP (500 mg/kg) (n = 15); (F) Schematic of mice receiving neutralizing peptide derived from human SAA1 (aa29-42) or the scramble peptide (50 μg/mice) one hour before APAP-induced mouse liver injury; (G) serum samples were collected for detection of ALT activities (n=3-4); (H) the liver tissues were collected for H&E staining (Scale bar = 100 μm), (I) quantification of necrosis areas in H&E staining sections (n=3-7). *, p < 0.05; ****, p < 0.001.

Article Snippet: Western blotting was performed to detect the protein level of Saa1/2 (cat. ab124964; Abcam), β-actin (cat.4970; Cell Signaling Technology) and Vcam-1 (cat.32653S; Cell Signaling Technology).

Techniques: Staining, Injection, Derivative Assay

Journal: bioRxiv

Article Title: Serum Amyloid A promotes Acetaminophen-induced liver injury by damaging sinusoidal endothelial cell and exacerbating platelet aggregation in liver

doi: 10.1101/2021.12.01.470869

Figure Lengend Snippet: LSECs were isolated from normal mice. (A) Flow cytometry was used to determine the proportion of dead LSECs induced by different concentrations of APAP (10, 20 or 30 mM) for 6 hours; (B) Quantification data of flow cytometry assay in (A) experiment (n=3); (C) Flow cytometry was used to determine the proportion of LSEC death induced by APAP (10 mM) or recombinant Saa1/2 proteins alone or mixed together; (D) Hoechst 33342 and PI double staining was used to measure the proportion of LSEC deaths induced by stimulating of APAP (10 mM) or recombinant Saa1/2 alone or combined together for 6 hours (scale bars = 50 μm); (E) Quantification data of double staining assay in (D) (n=3); (F) Cell morphology images of LSEC treated with APAP (10 mM) or Saa1/2 proteins alone or mixed together for 24 hours (scale bars = 50 μm). *, p < 0.05; **, p < 0.01.

Article Snippet: Western blotting was performed to detect the protein level of Saa1/2 (cat. ab124964; Abcam), β-actin (cat.4970; Cell Signaling Technology) and Vcam-1 (cat.32653S; Cell Signaling Technology).

Techniques: Isolation, Flow Cytometry, Recombinant, Double Staining

Journal: bioRxiv

Article Title: Serum Amyloid A promotes Acetaminophen-induced liver injury by damaging sinusoidal endothelial cell and exacerbating platelet aggregation in liver

doi: 10.1101/2021.12.01.470869

Figure Lengend Snippet: (A) Representative images of adhesive platelets (red color). LSECs were cultured for 48 h in vitro and treated with Saa1/2 (3 ug/mL) alone or mixed with Cu-CPT22 (1 μM) for 2 hours, then Dil-labeled platelets were added and incubated with LSEC for 1 hour (scale bars = 50 μm); (B) Quantitative results of adhesive platelets in (A) (n=3); (C) Q-PCR analysis of Vcam-1 mRNA level in LSEC (n=3); (D) Western blotting analysis of Vcam-1 in LSEC; (E) Western blotting analysis of Vcam-1 in liver tissues, that were collected from mice treated with IgG or anti-SAA neutralizing antibody before injection of APAP (300 mg/kg) for 6 hours; (F) Quantification of relative Vcam-1 expression in (E) (n=3); (G) Representative images of adhesive platelets (red color) to LSECs treated with recombinant Saa1/2 alone or together with anti-Vcam-1 neutralizing antibodies (scale bars = 50 μm); (H) Quantitative results of adhesive platelets in (G) (n=3). *, p < 0.05; **, p < 0.01.

Article Snippet: Western blotting was performed to detect the protein level of Saa1/2 (cat. ab124964; Abcam), β-actin (cat.4970; Cell Signaling Technology) and Vcam-1 (cat.32653S; Cell Signaling Technology).

Techniques: Cell Culture, In Vitro, Labeling, Incubation, Western Blot, Injection, Expressing, Recombinant

Journal: Journal of Inflammation Research

Article Title: LPS-Induced Inflammation Affects Midazolam Clearance in Juvenile Mice in an Age-Dependent Manner

doi: 10.2147/JIR.S321492

Figure Lengend Snippet: Data Comparison Between Experimental and Control Groups of Mice

Article Snippet: SAA1 levels were measured by ELISA (Cusabio, CSB-EL020656MO, Wuhan, China) in plasma samples.

Techniques: Comparison, Control

Journal: Journal of Inflammation Research

Article Title: LPS-Induced Inflammation Affects Midazolam Clearance in Juvenile Mice in an Age-Dependent Manner

doi: 10.2147/JIR.S321492

Figure Lengend Snippet: Covariate Analysis

Article Snippet: SAA1 levels were measured by ELISA (Cusabio, CSB-EL020656MO, Wuhan, China) in plasma samples.

Techniques: Selection